Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-Dependent Termination of Transcription In Vitro.

Cédric Nadiras (1) , Annie Schwartz (1) , Mildred Delaleau (1) , Marc Boudvillain (1)

1 CBM - Centre de biophysique moléculaire

Besides their well-known posttranscriptional effects on mRNA translation and decay, sRNAs and associated RNA chaperones (e.g., Hfq, CsrA) sometimes regulate gene expression at the transcriptional level. In this case, the sRNA-dependent machinery modulates the activity of the transcription termination factor Rho, a ring-shaped RNA translocase/helicase that dissociates transcription elongation complexes at specific loci of the bacterial genome. Here, we describe biochemical assays to detect Rho-dependent termination signals in genomic regions of interest and to assess the effects of sRNAs and/or associated RNA chaperones on such signals.

Domaines: Biochimie [q-bio.BM]; Biologie moléculaire; Biologie structurale [q-bio.BM]

Cédric Nadiras, Annie Schwartz, Mildred Delaleau, Marc Boudvillain. Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-Dependent Termination of Transcription In Vitro.. Methods in Mecular Biology, 1737, humana press, Totowa, NJ-USA, pp.99-118, 2018, Bacterial regulatory RNA (Arluison & Valverde, eds), ⟨10.1007/978-1-4939-7634-8_7⟩. ⟨hal-01880631⟩